Seminar: Bioinorganic Chemistry | May 20 | 10.00 a.m.-11.00 a.m. | Room 8.2.38
Giuseppe Grasso, Associate Professor of General and Inorganic Chemistry, Department of Chemical Sciences, University of Catania, Catania, Italy
Surface plasmon resonance to detect diffusion coefficients of molecules: entering a novel field of applications
Host: Ana Paula Paiva
We have recently developed a novel SPR-based method to measure the diffusion coefficient (D) of molecules with unprecedented precision and reproducibility.[1] Here, we applied this method to measuring the D value of model molecules (N-alcohols) and correlate it to the configurational entropy. In addition, protein involved in conformational diseases were also testes, monitoring how D is affected by pH and the presence of metal ions (copper and zinc). The reported SPR method, for the first time hereby applied to proteins, besides giving an insight into proteins oligomerization and aggregation phenomena, proved to be very robust for determining the D value of any biomolecule. A theoretical background is given together with the software description, specially designed to fit the experimental data. This new way of applying SPR represents an innovation in the bio-sensing field and expanding the potentiality of commonly used SPR instruments well over the canonical investigation of biomolecular interactions.
References:
1. Zingale, G.A.; Pandino, I.; Distefano, A.; Tuccitto, N.; Grasso G. Biosensors & Bioelectronics: X 2023, 13, 100306.